Journal: International Journal of Pharmaceutics: X

Article Title: Zein-based polysaccharide-tannic acid films as multifunctional barriers to prevent post-surgical adhesions

doi: 10.1016/j.ijpx.2026.100515

Figure Lengend Snippet: In vitro characterization: A) direct cells contact onto film surfaces and CLSM images after 6 days; indirect cell proliferation after 3 and 6 days towards: B) NHDF; C) Caco-2; D) CLSM images (in blue – nuclei; in green – cytoskeleton). (mean values ± SD; n = 3), ANOVA one-way; Scheffé test (* P value <0.05; ***P value <0.001). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The in vitro characterization was carried out using normal human dermal fibroblasts (NHDF) from juvenile foreskin (PromoCell, VWR, Milan, Italy) and Human colorectal adenocarcinoma cells (Caco-2).

Techniques: In Vitro

Journal: Biomolecules

Article Title: Mandelamide Isolated from Prunus persica Flowers Attenuates TNF-α–Driven Oxidative and Inflammatory Responses in Human Skin Cells

doi: 10.3390/biom16050672

Figure Lengend Snippet: Effects of the Prunus persica flower extract and compounds 1–4 on TNF-α–induced intracellular ROS production in human dermal fibroblasts (HDFs). Cells were pretreated with the indicated concentrations of the extract or compounds for 1 h, followed by stimulation with TNF-α (20 ng/mL) in the presence of DCFDA for 15 min. Intracellular ROS levels were quantified by measuring fluorescence intensity at excitation/emission wavelengths of 485/535 nm. Data are expressed as fold changes relative to the untreated control and presented as mean ± SEM ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test; ### p < 0.001 vs. control; *, **, and *** p < 0.05, p < 0.01, and p < 0.001 vs. TNF-α–treated group.

Article Snippet: Human dermal fibroblasts (HDFs; CAT No. C-12302, adult donor) and human epidermal keratinocytes (HEKs; CAT No. C-12006, pooled adult donor) were purchased from PromoCell GmbH (Heidelberg, Germany).

Techniques: Fluorescence, Control

Journal: Biomolecules

Article Title: Mandelamide Isolated from Prunus persica Flowers Attenuates TNF-α–Driven Oxidative and Inflammatory Responses in Human Skin Cells

doi: 10.3390/biom16050672

Figure Lengend Snippet: Effects of the Prunus persica flower extract and compounds 1 – 4 on TNF-α–induced MMP-1 secretion and type I collagen (COL1A1) production in human dermal fibroblasts (HDFs). Cells were pretreated with the indicated concentrations of the extract or compounds for 1 h, followed by stimulation with TNF-α (20 ng/mL) for 24 h. The secretion levels of ( A ) MMP-1 and ( B ) COL1A1 were quantified using ELISA. Data are expressed as mean ± SEM ( n = 2). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test; ### p < 0.001 vs. control; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. TNF-α–treated group. Quercetin (Que) was used as a positive control.

Article Snippet: Human dermal fibroblasts (HDFs; CAT No. C-12302, adult donor) and human epidermal keratinocytes (HEKs; CAT No. C-12006, pooled adult donor) were purchased from PromoCell GmbH (Heidelberg, Germany).

Techniques: Enzyme-linked Immunosorbent Assay, Control, Positive Control

Journal: Biomolecules

Article Title: Mandelamide Isolated from Prunus persica Flowers Attenuates TNF-α–Driven Oxidative and Inflammatory Responses in Human Skin Cells

doi: 10.3390/biom16050672

Figure Lengend Snippet: Radar plot analysis comparing the biological activities of Prunus persica flower extract and compounds 1 – 4 in TNF-α–stimulated human dermal fibroblasts (HDFs). The analysis was based on the inhibition of ROS production and MMP-1 secretion and the enhancement of COL1A1 expression. The axes represent the percentage of inhibition (for ROS and MMP-1) or recovery (for COL1A1) relative to the TNF-α–treated group. Activity was normalized based on the difference between the TNF-α–treated and untreated control groups. Semi-quantitative scores (0–4) were assigned based on the magnitude of biological response: 0 (<20%, minimal effect), 1 (20–39%, weak effect), 2 (40–69%, moderate effect), 3 (70–89%, strong effect), and 4 (≥90%, near-complete effect). A larger radar plot area indicates greater overall efficacy.

Article Snippet: Human dermal fibroblasts (HDFs; CAT No. C-12302, adult donor) and human epidermal keratinocytes (HEKs; CAT No. C-12006, pooled adult donor) were purchased from PromoCell GmbH (Heidelberg, Germany).

Techniques: Inhibition, Expressing, Activity Assay, Control

Journal: Biomolecules

Article Title: Mandelamide Isolated from Prunus persica Flowers Attenuates TNF-α–Driven Oxidative and Inflammatory Responses in Human Skin Cells

doi: 10.3390/biom16050672

Figure Lengend Snippet: Effects of mandelamide ( 4 ) on MAPK phosphorylation in TNF-α–stimulated human dermal fibroblasts (HDFs). ( A ) Cells were pretreated with mandelamide at the indicated concentrations (12.5, 25, 50, and 100 μM) for 1 h, followed by stimulation with TNF-α for 15 min. Phosphorylation and total protein levels of ERK, JNK, and p38 were analyzed by Western blotting, with GAPDH used as a loading control. ( B ) Quantitative data are expressed as fold changes relative to the untreated control and presented as mean ± SEM ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test; ### p < 0.001 vs. control; *** p < 0.001 vs. TNF-α–treated group. Original western blots can be found at .

Article Snippet: Human dermal fibroblasts (HDFs; CAT No. C-12302, adult donor) and human epidermal keratinocytes (HEKs; CAT No. C-12006, pooled adult donor) were purchased from PromoCell GmbH (Heidelberg, Germany).

Techniques: Phospho-proteomics, Western Blot, Control